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. 2019 Feb 19;19(4):3168–3178. doi: 10.3892/mmr.2019.9970

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — ASX inhibits TSP-1 expression in renal fibrotic tissue. (A) Expression of TSP-1 in murine renal tissues was assessed by immunohistochemical staining (magnification, ×600). (B) mRNA levels of TSP-1 were detected by reverse transcription-quantitative polymerase chain reaction. (C) Protein expression levels of TSP-1 were assessed by western blotting. The data are presented as the means ± standard deviation (n=6). *P<0.05, **P<0.01, ***P<0.001. ASX, Astaxanthin; TSP-1, thrombospondin 1; UUO, unilateral ureteral obstruction.