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. 2019 Feb 7;19(4):2749–2757. doi: 10.3892/mmr.2019.9944

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Copyright: © Qi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — mTOR and p62/SQSTM1 expression in the coronal pulp. (A) Images of immunofluorescence staining for mTOR (red), p62/SQSTM1 (green) and DAPI (blue) were captured using a fluorescence microscope (magnification, ×200; scale bar, 100 µm). (B) p62/SQSTM1 and (C) mTOR were significantly decreased in the irreversible pulpitis group on days 5 and 7 as compared with the day 0 group. The experiments were repeated in triplicate, and the results were analyzed using ImageJ software. Error bars represent the standard error of the mean. **P<0.01 and ***P<0.001 vs. day 0 group. mTOR, mammalian target of rapamycin; SQSTM1, sequestosome 1.