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. 2019 Feb 7;19(4):2749–2757. doi: 10.3892/mmr.2019.9944

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Copyright: © Qi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — ATG5, ATG7, LC3 and Beclin-1 expression in the radicular pulp. (A) Images of the immunofluorescence staining for ATG5 (red), ATG7 (red), LC3 (green), Beclin-1 (red) and DAPI (blue) were captured using a fluorescence microscope (days 1–7; magnification, ×200; scale bar, 100 µm). (B) ATG5, (C) ATG7, (D) LC3 and (E) Beclin-1 expression area was significantly increased in the irreversible pulpitis group on days 1–7 as compared with the day 0 group. The experiments were repeated in triplicate, and the results were analyzed using ImageJ software. Error bars represent the standard error of the mean. **P<0.01 and ***P<0.001 vs. day 0. ATG, autophagy-related; LC3, light chain 3.