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. 2019 Feb 19;19(4):3087–3094. doi: 10.3892/mmr.2019.9968

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — The expression levels of PADI4 in vitro and knockdown of PADI4 in A549 cells. (A) The statistical analysis represents the mRNA expression level of PADI4 in different lung cancer cells and normal lung epithelial cells. (B) The representative bands of western blotting and the statistical analysis. Differences among groups were analyzed by one-way ANOVA, followed by Bonferroni's multiple comparison test. *P<0.05 or **P<0.01 vs. BEAS-2B, ^#P<0.05 vs. H1299, n=3. (C) The efficiency of PADI4 knockdown by shRNA was confirmed by reverse transcription-quantitative polymerase chain reaction. (D) The efficiency of PADI4 knockdown by shRNA was confirmed by western blotting. Differences among groups were analyzed by one-way ANOVA, followed by Bonferroni's multiple comparison test. **P<0.01 vs. Ctrl, n=3. Ctrl, control; NC, negative control; sh, short hairpin; PADI4, protein-arginine deiminase type-4; ANOVA, analysis of variance.