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. 2019 Feb 14;19(4):3105–3113. doi: 10.3892/mmr.2019.9955

Figure 4.

Figure 4.

HASMC phenotypic transformations are regulated by Rab7 in vitro. (A) Western blotting revealed Rab7 downregulation in Case 4 HASMCs. Following Rab7 knockdown, α-SMA expression was notably increased and osteopontin levels were decreased compared with the control. ***P<0.001 vs. CON. (B) Overexpression of Rab7 promoted phenotypic transformation in Case 8 HASMC as indicted by alterations in the expression of α-SMA and osteopontin. 3-MA was used to inhibit autophagy; the expression of α-SMA and osteopontin was markedly altered in Rab7-overexpressing cells (Case 8). ***P<0.001, *P<0.05 vs. CON. 3-MA, 3-methyladenine; Ctrl, control; HASMC, human aortic smooth muscle cells; LC3, microtubule-associated protein light chain 3; siCtrl, small interfering RNA control; siRab7, siRNA Rab7.