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. 2019 Jan 30;19(4):2581–2590. doi: 10.3892/mmr.2019.9908

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Copyright: © Qin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — RP regulates the mitochondrial-associated gene expression. Cardiomyocytes were treated with MI/R, 20 µM RP+MI/R, 40 µM RP+MI/R and 80 µM RP+MI/R. (A) RT-qPCR and (B) western blot assays were performed on the expression levels of mitochondrial cyto c and mitochondrial AIF in cardiomyocytes. (C) RT-qPCR and (D) western blot assays were conducted to determine the expression levels of cytosolic cyto c and cytosolic AIF in cardiomyocytes. ^#P<0.05 vs. Control; *P<0.05, **P<0.01, ***P<0.001 vs. MI/R. Rp, rhynchophylline; MI/R, myocardial ischemia-reperfusion; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; cyto c, cytochrome c; AIF, apoptosis-inducing factor; COX, cyclooxygenase.