Figure 8.

Viability of yeast suspension and yeast biofilm as tested by fluorescent fluorescein diacetate/ethidium bromide staining. The green staining indicates living cells and the orange staining indicates dead cells. Staining of Candida albicans ATCC 10231 suspensions following incubation at (A) 37°C and (B) 85°C for 30 min. Staining of C. albicans ATCC 10231 biofilms harvested from the bottom of wells following incubation at 37°C in the (C) absence of farnesol (control) and (D) presence of 3 mM farnesol. Staining of C. albicans ATCC 10231 biofilms harvested from the bottom of wells following incubation at 37°C in the (E) absence of tyrosol (control) and (F) presence of 20 mM tyrosol. Scale bars, 50 µm