Figure 4.

Blocking CSF-1R inhibits proliferation of endometrial cancer cells. (A) Immunofluorescence staining of M1 macrophage (iNOS and CD86) and M2 macrophage (Arg-1 and CD206) in U937 cell lines, co-cultured with ECC-1/HEC-1A cell lines and treated with 100 U/ml M-CSF. (B) Cell counting kit-8 assay found that U937 cells could promote ECC-1 and HEC-1A cell proliferation. Additionally, the CSF-1R inhibitor PLX3397 (10 µM) inhibits proliferation of ECC-1 and HEC-1A cells in the co-culture system. (C) Immunofluorescence staining of Ki67 detecting EC cell proliferation. Data are presented as the mean ± standard deviation from 5 independent experiments; *P<0.05, **P<0.01 vs. Control. Scale bar: 50 µm. Arg, arginase; CD, cluster of differentiation; CSF, colony-stimulating factor; CSF-1R, colony-stimulating factor 1 receptor; EC, endometrial cancer; iNOS, inducible nitric oxide synthase.