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. 2019 Mar 18;18:1534735419833795. doi: 10.1177/1534735419833795

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Histological changes in the intestinal villi and tongue were determined using hematoxylin and eosin (H&E) staining. (A) The upper 3 pictures showed the longitudinal section of jejunum on 40× field, while the lower 3 pictures showed the structure on 100× field. (B and C) The villi length and crypt depth of intestinal villi were randomly measured in different parts of jejunum on the same group of samples. ***P < 0.001. (D) Cell lysates of intestinal samples were extracted, and the level of Bcl-2, Bax, and cleaved caspase 7 were analyzed by Western blot. Beta-actin was used as an internal control. (E) The upper 3 pictures showed the cross section of tongue on 40× field, while the lower 3 pictures showed the structure on 100× field. (F) The epithelial thickness of the tip tongue was measured. ***P < .001. Data presented are means ± SD.