Figure 5.
The effects of 5-fluorouracil (5-FU) and GMI (Ganoderma microsporum immunomodulatory protein) on the apoptosis of SAS and SCC9 cells. Apoptosis-related proteins were detected by Western blot after (A) SAS and (B) SCC9 cells (8 × 105 cells/60 mm dish) were treated with various concentrations of GMI and 5-FU for 48 hours. GMI was pretreated for 1 hour before 5-FU. Beta-actin was used as an internal control. (C) SAS and (D) SCC9 cells (8 × 105 cells/60 mm dish) were treated the same way as (A) and (B). The number of apoptotic cells were assessed by annexin-V and propidium iodide staining through flow cytometry.