Figure 3.

The nucleotide and deduced amino acid sequences of the A‐chain (A) and B‐chain (B) of SPLs. The N‐terminal amino acid sequence of B‐chain determined from the purified proteins is indicated by dotted lines. Based on the sequence of the DNA fragment amplified with two degenerate primers, DF1 and DR1, forward primer F1 was designed, and used for 3′‐RACE of SPL‐2 cDNA. The N‐terminal amino acid of the mature protein is numbered as “+1.” The primers used for PCR are indicated by horizontal arrows. An asterisk indicates the stop codon.