Figure 4.

Effect of IGF‐loop deletion on dissociation kinetics. (A) Dissociation kinetics for complexes of ClpP and different variants of single‐chain ClpX^ΔN were measured in 1 mM ATP by monitoring changes in BLI response following transfer of the biosensor into buffer lacking ClpP. (B) Half‐lives were calculated from single exponential fits of dissociation experiments like those shown in Panel A. The half‐life for the variant with six IGF loops is a lower limit. Values are averages (N = 3) ± SD.