Figure 6.

Effects of mutations in the IGF loop. (A) Rates of degradation of GFP‐ssrA (20 μM) by ClpP (0.9 μM) and ClpX^ΔN variants containing longer or shorter IGF loops (0.3 μM hexamer). (B) Rates of degradation of GFP‐ssrA (20 μM) by ClpP (0.9 μM) and ClpX^ΔN variants (0.3 μM hexamer) with single‐ or double‐residue substitutions in the IGF loop. (C) Association rate constants determined by BLI experiments using 1 μM ClpP and 2 mM ATP. (D) Dissociation half lives determined by BLI experiments in the presence of 2 mM ATP. In Panels C and D, single‐chain ClpX^ΔN variants had the IGF‐loop deleted from Subunit B and had a wild‐type or mutant IGF motif in Subunit A. In all panels, values are averages (N = 3) ± SD.