Figure 2.

(A–E) Neuronal autophagy was upregulated in the TBI models in vitro. (A) Cultured HT22 neurons with a scratch injury under the transmission light microscope. (B, C) Immunoblot analysis showed that LC3 II/I and Beclin-1 expressions were upregulated in cultured HT22 neurons after scratch injury, while P62 expression was downregulated after scratch injury. (D) Immunofluorescence staining showed upregulation of LC3 puncta in HT22 neurons after scratch injury. ** P<0.01, *** P<0.001 versus control group. TBI – traumatic brain injury.