Table 2.
A table showing the mass cytometry panel used to immunophenotype the blood of CRPS and healthy control participants. The columns represent the rare-earth metal isotype used for conjugation, species reactivity, target antigen, clone, manufacturer and staining concentration. The antibody from Fluidigm was purchased metal labelled, and antibodies from all other manufacturers were purchased, metal-conjugated and validated in-house by the Ramaciotti Facility for Human Systems Biology
| Metal isotype | Reactivity | Antigen | Clone | Manufacturer | Staining conc. (μg/ml) | Staining step buffer |
|---|---|---|---|---|---|---|
| 110Pd | Mouse, Human | CD45 | 30-F11 | BD | 6 | FACS |
| 115In | Human | CD8 | RPA-T8 | Biolegend | 4 | FACS |
| 141Pr | Human | CD235ab | HIR2 | Biolegend | 1.6 | FACS |
| 142Nd | Human | CD19 | HIB19 | BD | 1 | FACS |
| 143Nd | Human | CD56 | REA196 | Miltenyi Biotec | 0.5 | FACS |
| 144Nd | Human | TCR γδ | B1 | BD | 2 | FACS |
| 145Nd | Human | CD4 | RPA-T4 | BD | 0.5 | FACS |
| 146Nd | Human | DAP12 | 406288 | R&D systems | 1 | Perm. buffer |
| 147Sm | Human, Mouse | TREM-2 | 237920 | R&D systems | 8 | FACS |
| 148Nd | Human | CD16 | 3G8 | BD | 1.5 | FACS |
| 149Sm | Human | CD25 | 2A3 | BD | 1 | FACS |
| 150Nd | Mouse, Human | pSTAT5 [pY694] | 47 | BD | 1.5 | Methanol |
| 151Eu | Human | CD123 (IL-3R) | 6H6 | Biolegend | 1 | FACS |
| 152Gd | Human | CD66b | 80H3 | Fluidigm | 3 (μl/ml) | FACS |
| 153Eu | Mouse, Human | pSTAT1 [pY694] | 14/P-Stat1 | BD | 2 | Methanol |
| 154Gd | Mouse, Human | pAKT [T308] | J1.233.371 | BD | 4 | Methanol |
| 155Gd | Human | CD354 (TREM-1) | TREM-37 | Biolegend | 2 | FACS |
| 156Gd | Human | pp38 MAPK [T180/Y182] | 30/p38 MAPK | BD | 4 | Methanol |
| 158Gd | Human, Mouse | pSTAT3 [Y705] | 4/P-STAT3 | BD | 20 | Methanol |
| 159Tb | Human | CD197 (CCR7) | G043H7 | Biolegend | 8 | FACS |
| 160Gd | Human | CD14 | M5E2 | Biolegend | 6 | FACS |
| 161Dy | Human | CD141 (BDCA3) | AD5-14H12 | Miltenyi Biotec | 8 | FACS |
| 162Er | Human | Foxp3 | PCH101 | eBioscience | 3 | Perm. buffer |
| 163Dy | Human | CD1c | L161 | Biolegend | 1 | FACS |
| 164Er | Human | CD45RO | UCHL1 | Biolegend | 6 | FACS |
| 165Ho | Human | CD61 (Integrin β 3) | VI-PL2 | Biolegend | 1 | FACS |
| 166Er | Human | pp65 (NFκB) [S529] | K10-895.12.50 | BD | 4 | Methanol |
| 167Er | Human | CD11c | Bu15 | Biolegend | 1 | FACS |
| 168Er | Human | pERK 1/2 [Y202/204] | 4B11B69 | Biolegend | 2 | Methanol |
| 169Tm | Human | CD45RA | HI100 | Biolegend | 2 | FACS |
| 170Er | Human | CD3 | UCHT1 | BD | 2 | FACS |
| 171Yb | Human | Arginase-1 | 14D2C43 | Biolegend | 4 | Perm. buffer |
| 172Yb | Human | CD130 | AM64 | BD | 8 | FACS |
| 173Yb | Human, Mouse | pMAPKAPK-2 [T334] | P24-694 | BD | 4 | Methanol |
| 174Yb | Human | HLA-DR | G46-6 | BD | 2 | FACS |
| 175Lu | PE | PE | PE001 | Biolegend | 4 | FACS |
| 176Lu | Human | CD127 | A019D5 | Biolegend | 2 | FACS |
| 209Bi | Human, Mouse | T-bet | 4B10 | BD | 1 | Perm. buffer |
| n/a | Human | pPLC γ 2 [Y759]-PE | K86-689.37 | BD | 200 (μl/ml) | Methanol |