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. 2019 Mar 18;10:100. doi: 10.1186/s13287-019-1209-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Characterization of fMSC-iMSCs, aMSC- iMSCs, and ESC-iMSCs. a Morphology of iMSCs compared to native MSCs. b Flow cytometry-based analysis of MSC surface marker in iMSCs. Blue/Purple: iMSCs labeled with antibody specific to marker. Gray: isotype control. c Differentiation potential of iMSCs in vitro. Osteogenic: Alizarin Red S staining; adipogenic: Oil red O staining; chondrogenic: Alcian Blue staining. d Gene expression of MSC, hematopoietic, and pluripotency markers in iMSCs compared to primary MSCs and ESC H1. Representative images of n = 3 experiments