Fig. 3.

Emodin attenuates NETs and changes cytokines and ROS during urethane-induced lung carcinogenesis. a NETs in lung sections indicated by Cit-H3 examined by immunohistochemistry (n = 5, 40 ×). b NETs in lung sections indicated by PAD4 examined by immunohistochemistry (n = 5, 40 ×). c Cytokine levels in alveolar cavity examined by ELISA (n = 6). d ROS in alveolar cavity examined by ELISA (n = 6). The data present Mean ± SD, the experiments were repeated 3 times, and statistical significance was determined by a t-test. *P < 0.05, **P < 0.01 vs control