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. 2019 Mar 8;10:231. doi: 10.3389/fphys.2019.00231

FIGURE 3.

FIGURE 3

Effects of fecal microbiota transplantation (FMT) on ROS production and NADPH oxidase pathway in the brain PVN. CM-H2DCFDA-detected intracellular ROS in absence and presence of NADPH oxidase inhibitor apocynin (50 μM) (A) and NADPH oxidase activity measured by DHE fluorescence measured in the microplate reader (B) in homogenates from brain PVN. mRNA levels of NADPH oxidase subunits NOX-1, NOX-4, p47phox and p22phox (C) in the brain PVN from all experimental groups. Strain factor, FMT factor and I interaction between strain and FMT factors. ++p < 0.01 and ns (not significant) for the probability based on a two-way analysis of variance. Values are means ± SEM (n = 5–8). ∗P < 0.05 and ∗∗P < 0.01 vs. WKY with stool transplant from WKY (W-W); #P < 0.05 vs. SHR with stool transplant from SHR (S-S), statistical significance for the probability based on a Sidak’s correction multiple comparisons test.