Table 1.
Oligonucleotides for real-time RT-PCR.
| mRNA targets | Descriptions | Sense | Antisense |
|---|---|---|---|
| NOX-1 | NOX-1 subunit of NADPH oxidase | TCTTGCTGGTTGACACTTGC | TATGGGAGTGGGAATCTTGG |
| NOX-4 | NOX-4 subunit of NADPH oxidase | ACAGTCCTGGCTTACCTTCG | TTCTGGGATCCTCATTCTGG |
| p22phox | p22phox subunit of NADPH oxidase | GCGGTGTGGACAGAAGTACC | CTTGGGTTTAGGCTCAATGG |
| p47phox | p47phox subunit of NADPH oxidase | CCCAGCGACAGATTAGAAGC | TGGATTGTCCTTTGAGTCAGG |
| TNF-α | Tumor necrosis factor-alpha | ACGATGCTCAGAAACACACG | CAGTCTGGGAAGCTCTGAGG |
| IL-6 | Interleukin-6 | GATGGATGCTTCCAAACTGG | AGGAGAGCATTGGAAGTTGG |
| IL-10 | Interleukin-10 | GAATTCCCTGGGAGAGAAGC | GCTCCACTGCCTTGCTTTTA |
| IL-17a | Interleukin-17a | CTTCACCTTGGACTCTGAGC | TGGCGGACAATAGAGGAAAC |
| IFNγ | Interferon gamma | GCCCTCTCTGGCTGTTACTG | CCAAGAGGAGGCTCTTTCCT |
| cd11b | Cd11b | GAGAACTGGTTCTGGCTTGC | TCAGTTCGAGCCTTCTT |
| CCL2 | C-C chemokine ligand 2 | CCTCCACCACTATGCAGGTC | CAGCCGACTCATTGGGATCA |
| Olfr59 | Olfactory receptor 59 | CTGCTAGTCATGGGTGTAGATG | CAAGGGTGATAGAACGGTAAGG |
| GPR-41 | G-protein-coupled receptor-41 | TGACGGTGAGCATAGAACGTTT | GCCGGGTTTTGTACCACAGT |
| GPR-43 | G-protein-coupled receptor-43 | TCGTGGAAGCTGCATCCA | GCGCGCACACGATCTTT |
| Occludin | Occludin | AGCCTGGGCAGTCGGGTTGA | ACACAGACCCCAGAGCGGCA |
| Muc2 | Mucin-2 | CGATCACCACCATTGCCACTG | ACCACCATTACCACCACCTCAG |
| ZO-1 | Zonula occludens-1 | GCCAGCCAGTTCCGCCTCTG | AGGGTCCCGGGTTGGTG |
| IL-1β | Interleukin-1 beta | GTCACTCATTGTGGCTGTGG | GCAGTGCAGCTGTCTAATGG |
| TH | Tyrosine hydroxylase | GATTGCTACCTGGAAGGAGGT | AGTCCAATGTCCTGGGAGAAC |
| GAPDH | Glyceraldehyde-3-Phosphate Dehydrogenase | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA |