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. 2019 Mar 11;10:454. doi: 10.3389/fmicb.2019.00454

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Copyright © 2019 Liu, Xue and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of data preprocessing on resolving the differences of microbial communities. (A) The overview of pairwise Bray-Curtis dissimilarity of bacterial and eukaryotic plankton communities based on five different DNA extraction kits after the OTUs with < = 1, 5, 10, 50, 100, 500 sequences were removed. For bacteria, linear regression resulted in a significant pattern for between kits (R² = 0.19, P < 0.01) and within kits (R² = 0.14, P < 0.01). For eukaryotes, linear regression resulted in a significant pattern only for between kits (R² = 0.01, P < 0.01). (B) The overlap of three replicates of bacterial and eukaryotic plankton communities based on five different DNA extraction kits after the OTUs with < = 1, 5, 10, 50, 100, 500 sequences were removed. Linear regression resulted in a significant correlation for bacteria (R² = 0.37, P < 0.01) and eukaryotes (R² = 0.45, P < 0.01).