Table 2.

Analysis of similarity (ANOSIM) and permutational multivariate analysis of variance (PERMANOVA) for comparisons of microbial plankton communities among five different DNA extraction kits based on Bray-Curtis dissimilarity.

	ANOSIM				PERMANOVA
Category	Bacteria		Eukaryotes		Bacteria		Eukaryotes
	R	P-value	R	P-value	R2	P-value	R2	P-value
All	0.34	0.004	0.32	0.001	0.33	0.006	0.37	0.002
AT	0.48	0.001	–	–	0.50	0.009	–	–
CAT	0.40	0.001	0.51	0.001	0.30	0.318	0.54	0.001
CRAT	0.26	0.008	0.32	0.002	0.40	0.073	0.37	0.002
CRT	0.28	0.005	0.33	0.003	0.33	0.150	0.38	0.005
MT	0.58	0.001	0.18	0.040	0.39	0.016	0.35	0.241
RT	0.35	0.001	0.26	0.002	0.31	0.003	0.34	0.002

Bold font indicates the significance at P < 0.05. Values show the R and R² values for ANOSIM and PERMANOVA, respectively. The operational taxonomic units (OTUs) were defined at 97% sequence similarity threshold. The ANOSIM statistic compares the mean of ranked dissimilarities among groups to the mean of ranked dissimilarities within groups. An R value close to “1” suggests dissimilarity between groups, while an R value near “0” suggests an even distribution of high and low ranks within and between groups. Negative R values indicate that dissimilarities are greater within groups than between groups. AT, always abundant taxa; CAT, conditionally abundant taxa; CRAT, conditionally rare and abundant taxa; CRT, conditionally rare taxa; MT, moderate taxa; RT, always rare taxa. Note that no always abundant taxa (AT) was identified for eukaryotic plankton in this study.