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. Author manuscript; available in PMC: 2019 Mar 19.
Published in final edited form as: Nature. 2018 Jul 25;560(7718):372–376. doi: 10.1038/s41586-018-0360-3

Figure 3. Inhibition of TM7SF2 and EBP is a unifying mechanism for many small-molecule enhancers of oligodendrocyte formation.

Figure 3.

a) Quantitation of sterol levels in OPCs treated with the indicated molecules at 2 μM (one well per condition; for validation in a second derivation of OPCs, see Extended Data Fig. 6). (b) Quantitation of sterol levels in OPCs treated with the indicated previously-reported enhancers of oligodendrocyte formation (n = 2 wells per condition except DMSO, n = 6). Representative of two independent experiments. c) Quantitation of EBP enzymatic activity in a biochemical assay. All treatments 10 μM. n = 3 independent enzymatic assays, except DMSO, n = 5. Bars indicate mean; error bars indicate standard deviation. p-Fluorohexahydro-sila-difenidol abbreviated as Sigma H127.