Table 3A:
Effect of delayed cell processing on immunophenotyping of T cell subsets using EDTA whole blood
| Reference (WB-D0) |
Delayed cell processing | P for trend | |||
|---|---|---|---|---|---|
| Cell Subsets# |
0hr Mean (95% CI) |
24hr Mean (95% CI) |
48hr Mean (95% CI) |
72hr Mean (95% CI) |
|
| Cell Viability | 93.0 (91.9, 94.1) |
81.1 *** (75.9, 86.3) |
80.4 *** (77.4, 83.3) |
88.3 *** (85.8, 90.8) |
0.07 |
| T cells | 73.1 (69.6,76.7) |
75.5 (71.1,79.9) |
74.6 (70.5,78.6) |
71.0 (65.9, 76.1) |
0.11 |
| Helper T cells | 63.1 (56.2, 69.9) |
64.2 (58.9, 69.6) |
65.4 (60.2, 70.6) |
62.7 (56.5, 68.9) |
0.88 |
| Helper T cells: CM | 41.5 (36.2, 46.8) |
39.3 (33.1, 45.4) |
40.2 (34.5, 45.8) |
39.9 (31.8, 48.0) |
0.09 |
| Helper T cell s: EFF | 0.4 (0.0, 0.8) |
0.5 (0.1, 0.9) |
0.4 (0.0, 0.7) |
0.8 (0.1, 1.5) |
0.1 |
| Helper T cells: EM | 18.6 (13.8, 23.3) |
22.8 (16.1, 29.4) |
20.7 (16.6, 24.7) |
21.3 (12.7, 29.9) |
0.29 |
| Helper T cells: Naïve | 36.3 (28.8, 43.7) |
34.2 (26.3, 42.1) |
36.4 (29.0, 43.8) |
34.6 (25.3, 43.9) |
0.94 |
| Cytotoxic T cells | 28.3 (23.7, 33.0) |
28.6 (23.9, 33.3) |
28.6 (24.0, 33.3) |
31.0 (25.3, 36.7) |
0.14 |
| Cytotoxic T cells: CM | 9.5 (5.7, 13.3) |
10.4 (6.5, 14.2) |
10.8 (6.7, 14.9) |
11.9 (7.8, 16.0) |
0.15 |
| Cytotoxic T cells: EFF | 21.1 (9.9, 32.3) |
24.1 (13.6, 34.6) |
25.4 (14.8, 35.9) |
27.7 (15.4, 40.0) |
0.01 |
| Cytotoxic T cells: pE | 49.5 (38.1, 70.0) |
52.3 (41.5, 63.0) |
51.1 (40.3, 61.9) |
49.8 (38.7, 60.8) |
0.38 |
| Cytotoxic T cells:pE1 | 16.9 (10.1, 23.6) |
15.5 (11.1, 20.0) |
14.5 (9.8, 19.3) |
14.1 (9.1, 19.1) |
0.17 |
| Cytotoxic T cells:pE2 | 31.3 (22.3, 40.4) |
29.5 (20.8, 38.3) |
31.9 (23.1, 40.8) |
33.7 (24.9, 42.4) |
0.92 |
| Cytotoxic T cells: EM | 39.2 (29.9, 48.5) |
37.3 (28.4, 46.3) |
35.3 (26.4, 44.3) |
33.8 (23.9, 43.6) |
0.002 |
| Cytotoxic T cells:EM1 | 62.7 (54.0, 71.4) |
62.7 (54.1, 71.2) |
60.0 (52.1, 67.8) |
55.0 (45.1, 64.8) |
0.001 |
| Cytotoxic T cells: EM2 | 12.2 (9.7, 14.6) |
11.7 (9.1, 14.3) |
13.1 (10.2, 16.0) |
16.1*** (13.3, 19.0) |
0.001 |
| Cytotoxic T cells: EM3 | 18.8 (10.6, 27.0) |
18.0 (10.1, 26.0) |
18.7 (10.9, 26.5) |
21.4 (11.8, 31.0) |
0.09 |
| Cytotoxic T cells: EM4 | 6.3 (4.6, 8.1) |
7.6 (5.3, 9.9) |
8.3 (6.5, 10.0) |
7.5 (5.3, 9.7) |
0.06 |
| Cytotoxic T cells:Naïve | 20.9 (13.8, 28.0) |
21.0 (14.1, 27.9) |
21.2 (14.6, 27.9) |
19.5 (11.2, 27.9) |
0.84 |
Indicates statistically significant pairwise comparisons (p<0.007) between WB-DO (reference) and the individual time points.
Blood collected in EDTA tube and processed at time o (WB-D0) was used as the reference for all comparisons
CD4+ and CD8+ subsets of T-cells were expressed as percentage of T-cells. Subsets of CD4+ and CD8+ cells (effector, effector memory, central memory and naïve cells) were expressed as percentage of CD4+ and CD8+ cells respectively. The cytotoxic pE, pE1 and pE2 subsets were expressed as a percentage of cytotoxic effector cells. Cytotoxic EM1, EM2, EM3 and EM4 subsets were expressed as a percentage of cytotoxic effector memory cells.