Table 4A:
Effect of delayed cell processing on immunophenotyping of subsets of B cells, NK cells, dendritic cells and monocytes using EDTA whole blood
| Reference (WB-D0) |
Delayed cell processing+ | ||||
|---|---|---|---|---|---|
| Cell Subsets# |
0hr Mean (95% CI) |
24hr Mean (95% CI) |
48hr Mean (95% CI) |
72hr Mean (95% CI) |
P for trend |
| B Cells | 6.8 (5.4,8.1) |
7.9 (6.2,9.5) |
8.8 ***
(7.0,10.5) |
9.1 (6.6, 11.5) |
0.01 |
| IgD+ memory B cells | 17.6 (13.4, 21.9) |
19.1 (13.5, 24.7) |
14.1 (10.8, 17.3) |
20.3 (15.2, 25.3) |
0.92 |
| IgD− memory B cells | 10.1 (7.7, 12.6) |
12.5*** (9.6, 15.4) |
17.2 *** (13.8, 20.6) |
11.18 (8.3, 14.1) | 0.05 |
| Naïve B cells | 66.5 (60.3, 72.8) |
61.3 *** (55.2, 67.4) |
57.7 *** (52.5, 63.0) |
63 (55.1, 70.9) |
0.02 |
| NK Cells | 9.7 (7.7,11.6) |
10.8 (8.6,12.9) |
10.7 (8.0,13.4) |
13.0 (10.1, 15.9) |
0.001 |
| NK cells CD56HI | 2.0 (1.4, 2.5) |
1.9 (1.4, 2.5) |
2.1 (1.3, 2.9) |
1.6 (1.1, 2.1) |
0.39 |
| NK cells CD56LO | 76.8 (67.3, 86.3) |
76.4 (67.7, 85.0) |
76.1 (67.1, 85.2) |
78.2 (69.0, 7.3) |
0.34 |
| Dendritic Cells | 1.9 (1.3,2.6) |
1.8 (1.3,2.3) |
1.7 (1.2,2.1) |
1.8 (1.3, 2.3) |
0.27 |
| DC-M | 79.9 (76.8,83.0) |
82.2 (79.4, 85.0) |
82.1 (78.3, 85.9) |
84.1 (80.4, 87.7) |
0.006 |
| DC-P | 9.7 (7.9, 11.5) |
7.1*** (5.4, 8.8) |
4.3*** (3.0, 5.6) |
3.6 *** (2.8, 4.4) |
<0.001 |
| Monocytes | 5.9 (3.0,8.9) |
5.5 (2.9,8.2) |
5.5 (3.3,7.8) |
5.1 (3.6, 6.7) |
0.68 |
| CD16− Monocytes | 92.5 (90.9, 94.0) |
92.7 (91.6, 93.7) |
91.9 (90.4, 93.5) |
91.5 (89.9, 93.1) |
0.14 |
| CD16+ Monocytes | 6.5 (5.1, 7.9) |
6.0 (5.1, 7.0) |
6.3 (5.1 7.6) |
6.6 (5.4, 7.9) |
0.72 |
***
Indicates statistically significant pairwise comparisons (p<0.007) between WB-DO (reference) and the individual time points
+
Blood collected in EDTA tube and processed at time o (WB-D0) was used as the reference for all comparisons
#
Subsets of B cells, monocytes, DCs and NK cells were expressed as a percentage of monocytes, DCs and NK cells respectively.