Table 4B:
Effect of cryopreservation alone and combined effect of delayed cell processing and cryopreservation on immunophenotyping of subsets of B cells, NK cells, dendritic cells and monocytes using blood collected in CPT™ tubes
| Reference (WB-D0) |
Cryopreser vation (PBMC- D0)+ |
Delayed cell processing and Cryopreservation+ |
||||
|---|---|---|---|---|---|---|
| Cell Subsets# |
0hr Mean (95% CI) |
0hr Mean (95% CI) |
24hr Mean (95% CI) |
48hr Mean (95% CI) |
72hr Mean (95% CI) |
P for Trend |
| B cells | 6.8 (5.4, 8.1) |
5.3 (4.5, 6.2) |
11.8*** (9.6, 13.9) |
15.0 *** (11.4, 18.6) |
7.1 (4.8, 9.5) |
0.46 |
| IgD+ memory B cells | 17.6 (13.4, 21.9) |
15.0 (12.1, 17.8) |
12.0 *** (9.5, 14.5) |
12.3 *** (9.7, 14.8) |
13.9*** (10.7, 17.2) |
0.01 |
| IgD− memory B cells | 10.1 (7.7, 12.6) |
11.7 (7.5, 15.9) |
9.6 (5.6, 13.5) |
9.9 (6.7, 13.1) |
9.7 (7.1, 12.2) |
0.77 |
| Naïive B cells | 66.5 (60.3, 72.8) |
68.2 (62.0, 74.4) |
71.7 (65.6, 77.8) |
68.4 (62.3, 74.4) |
66.1 (59.4, 72.9) |
0.40 |
| NK cells | 9.7 (7.7, 11.6) |
9.1 (6.5, 11.6) |
6.5*** (4.6, 8.4) |
4.0*** (2.6, 5.3) |
2.1*** (1.1, 3.1) |
<0.0001 |
| NK cells CD56HI | 2.0 (1.4, 2.5) |
1.6 (0.9, 2.4) |
1.4 (0.9, 1.9) |
1.5 (0.8, 2.2) |
0 9*** (0.4, 1.4) |
0.001 |
| NK cells CD56LO | 76.8 (67.3, 86.3) |
89.5 *** (85.2, 93.7) |
90.4 *** (85.7, 95.1) |
72.8 (63.3, 82.3) |
73.5 (64.0, 83.0) |
0.05 |
| Dendritic cells | 1.9 (1.3, 2.6) |
2.0 (1.6, 2.2) |
3.2 (2.4, 4.0) |
3.4 (1.9, 4.9) |
1.6 (1.3, 2.0) |
0.84 |
| DC-M | 79.9 (76.8,83.0) |
77.7 (68.8, 86.6) |
79.1 (73.5, 84.7) |
79.1 (74.2, 84.1) |
73.5 (66.0, 81.1) |
0.06 |
| DC-P | 9.7 (7.9, 11.5) |
12.2 (8.3, 16.1) |
14.9 (10.5, 19.2) |
13.6 (9.6, 17.7) |
16.6*** (12.1, 21.0) |
0.002 |
| Monocytes | 5.9 (3.0, 8.9) |
9.2 (6.7,11.8) |
14.5*** (10.5, 18.5) |
14.5*** (10.0, 19.0) |
6.4 (4.1, 8.7) |
0.85 |
| CD16− Monocytes | 92.5 (90.9, 94.0) |
93.9 (92.7, 95.1) |
96.3*** (95.7, 96.9) |
89.8 (84.7, 95.0) |
83.4 (74.2, 92.5) |
0.004 |
| CD16+ Monocytes | 6.5 (5.1, 7.9) |
4.7 (3.7, 5.6) |
2.5*** (2.1, 3.0) |
5.0 (1.76, 8.4) |
10.7 (2.3, 19.1 |
0.13 |
***
Indicates statistically significant pairwise comparisons (p<0.007) between WB-DO (reference) and the individual time points.
+
Blood collected in EDTA tube and processed at time o (WB-D0) was used as the reference for all comparisons.
#
Subsets of B cells, monocytes, DCs and NK cells were expressed as a percentage of monocytes, DCs and NK cells respectively.