Figure 1.

Schematic of Monomers in a 96-Well PCR Plate

Each monomer was prepared by PCR amplification by using monomer plasmids included in an Addgene kit as a template and different primers designed to amplify variant monomers (Table S1). In total, there were 60 base-determinant monomers and 2 linker monomers (dsDNA_10.5 and dsDNA_17.5). The position of each monomer is indicated in the well. The monomer plate can be easily regenerated by 96-well PCR amplification. This plate also contains two TALE backbone vectors, TALE-VP64 and TALE-VPR, which can be produced by E. coli DH5α transformation and extraction and added to the wells.