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Pipeline for Constructing a TALE with New Monomers
Steps for the construction of a TALE are outlined. Time spent on each step is given. It can be seen that the whole TALE assembly procedure can be finished in 1 day. After a colony PCR screening, plasmid extraction, and EcoRI digestion confirmation, the usable final TALE can be obtained on the second day. The time taken by the process is identical to that taken by the current CRISPR.
