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. 2019 Feb 28;18:137–145. doi: 10.1016/j.jare.2019.02.005

Fig. 1.

Fig. 1

Cloning, Expression, and Purification of ADH. (A) PCR amplification of C. albicans ADH, fragment size 1047 bp. (B) Map of double-digested recombined plasmid of ADH. Lane 1, the product of PCR amplification; Lane 2, NdeI and XhoI double-digested pET30a vector; Lane 3, NdeI and XhoI double-digested recombined plasmid; Lane M, molecular mass standards. (C) Expression and purification of ADH. Left: Lane 1, the whole lysis of E. coli BL21 induced by IPTG; Lane 2, the lysis precipitate; Lane 3, lysis supernatant; and Lane M, protein marker. Right: purified recombinant ADH, 36 kDa.