Figure 6.

ZIKV Induces Apoptosis by a PARP-Dependent Caspase-Dependent Mechanism

Six-week old Ifnar1^−/− or Ifnar1^−/−Axl^−/− mice were subcutaneously infected via footpad injection with 10⁵ plaque-forming unit Cambodian strain of ZIKV.

(A–J) At day 6, when mice begin to show viral pathogenesis, they were sacrificed, brains were collected in radioimmunoprecipitation assay (RIPA) buffer with protease inhibitor, and a western blot was performed. Membrane was probed with antibodies toward proteins involved in apoptosis. Protein expressions of (A) PARP, (B) cleaved PARP, (C) caspase 3, (D) cleaved caspase 3, (E) caspase 8, (F) caspase 9, (G) cleaved caspase 9, (H) caspase 12, and (I) cleaved caspase 12 were quantified using ImageJ. Data shown are representative of at least two independent experiments. Significance was tested with two-tailed Mann-Whitney U test. Error bars represent SEM (n = 4/group for each genotype).