Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 1;13:351–370. doi: 10.1016/j.isci.2019.02.026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

C/EBPβ-Interacting Multi-protein Complexes in HEK293T Cells

(A) Co-immunoprecipitation of FLAG-tagged C/EBPβ and the MLL/Ash2/Wdr5 complex.

(B) Left panel: FLAG-tagged C/EBPβ was co-expressed with GFP-tagged Spt16 and SSRP1. Right panel: Immunoprecipitation of FLAG-tagged C/EBPβ co-precipitates both endogenous FACT complex subunits.

(C) Interaction with the super elongation complex. Immunoprecipitation of human C/EBPβ (C/EBPβ) pulls down the entire super elongation complex.

(D) Interaction with the NuRD complex. The integral subunits of the NuRD complex MBD2 (HA-tagged) and MBD3 (GFDP-tagged) were pulled down by immunoprecipitation. Detection by western blot shows that C/EBPβ interacts with both subunits. IB, immunoblotting; IP, immunoprecipitation.