Skip to main content
. Author manuscript; available in PMC: 2019 Nov 12.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Oct 18;57(46):15040–15044. doi: 10.1002/anie.201806483

Figure 4.

Figure 4.

(a) LN229 cellular uptake and localization of labeled vesicles released from Sequogel. Labeled vesicles encapsulate FITC-dextran (FD, green) within their aqueous core and contain Rhodamine lissamine-PE (red) in their lipid bilayer. (b) Mechanism of cellular uptake of DOX-loaded vesicles released from Sequogel. Cells were incubated with Sequogel for 1 h with and without endocytic inhibitors. Mean fluorescence intensities of DOX under each inhibition condition were compared to those in absence of inhibitor to determine statistical significance, indicated by * for p < 0.05. (c) Representative histograms from flow cytometric detection of DOX internalization 4 h and 16 h post-incubation with Sequogel. (d) Live-cell imaging of LN229 cells after addition of Sequogel containing DOX vesicles and ERL at 16 h post-incubation.