Figure 4. in-situ time dependent SMFS during pDA formation.
a, Schematic of the in-situ experiments whereby the AFM liquid cell was filled with freshly prepared monomer solution (2 mg/ml dopamine.HCl in 100 mM bicine buffer at pH 8.5) and force spectroscopy was performed during the first two hours of pDA formation b, Representative F-D traces collected during in-situ polymerization of pDA; after two hours, particles formed in the solution and possibly also pDA deposition on the cantilever mirror surfaces interfered with the reflection of the laser light, making further measurements impossible.