PLS-DA of mutant III-4 (A) or mutant IV-22 (B) relative to the wild-type parent strain M129, and of mutant III-4 relative to mutant IV-22. Stars, M129; red squares, mutant III-4; blue squares, mutant IV-22. Cross-validated statistics were obtained using Venetian blinds with 10 data splits to represent the prediction performance for each model. CV, cross-validated; RMSECV, root mean square error, cross-validated.