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. Author manuscript; available in PMC: 2019 Mar 19.
Published in final edited form as: Cell Rep. 2018 Dec 11;25(11):2992–3005.e5. doi: 10.1016/j.celrep.2018.11.056

Figure 1. Aged hematopoietic stem cells exposed to inflammatory signals demonstrate increased myeloid output in a cell-intrinsic manner.

Figure 1.

(A) Schematic overview of the approach. (B)-(E) Young and aged mice were exposed to a second dose of LPS 1 month after the initial LPS challenge and the frequency of (B) CD11b+, (C) Gr-1+, (D) CD3+, and (E) CD19+ was observed for 9 weeks. (F)-(I) LT-HSCs sorted from young and aged CD45.2 mice were stimulated with LPS and Pam3csk4 for 2 hours prior to competitive transplant into CD45.1 recipients. Peripheral blood CD45.2 (F) CD11b+, (G) Gr-1+, (H) CD3+ and (I) CD19+ frequencies were measured by flow cytometry at 3 months post-reconstitution (n = 11–12 per group). Data represent at least two independent experiments and are presented as mean ± SEM. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001. P-values were corrected for multiple hypothesis testing by Bonferroni’s multiple comparison test.