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. Author manuscript; available in PMC: 2019 Mar 19.
Published in final edited form as: Cell Rep. 2018 Dec 11;25(11):2992–3005.e5. doi: 10.1016/j.celrep.2018.11.056

Figure 5. CD61 expression on LT-HSCs is a marker for mLT-HSCs.

Figure 5.

(A) Flow cytometry plots showing CD61 expression in aged and young LT-HSCs. (B)-(E) Total LT-HSCs, and those with elevated (CD61-high) and decreased (CD61-low) expression of CD61, from aged and young mice were transplanted into lethally irradiated young C57BL/6 recipient mice. Mice were subsequently challenged with a single dose of LPS as in Figure 1. Shown are the peripheral blood CD11b+ cell frequencies from mice transplanted with (B) young and (C) aged donor LT-HSCs, and the peripheral blood Gr-1+ frequencies from mice transplanted with (D) young and (E) aged donor LT-HSCs. (F)-(K) Transplanted mice were harvested at 4-months post-reconstitution. Shown are the bone marrow frequencies of (F) LT-HSCs, (G) CD61+ LT-HSCs, (H) CLPs, (I) GMPs, (J) CMPs and (K) MEPs in these mice. (A) CD61 high frequencies and SD are depicted for aged (red) and young (blue) (n=4 per group). (B-K) Data represent at least two independent experiments (n=7–12 per group) and are presented as mean ± SEM. ^ denotes p<0.05 for CD61-high vs. control, vdenotes p<0.05 for CD61-low vs. control using two way ANOVA. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001. P-values was corrected for multiple hypothesis testing by Bonferroni’s method.