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. Author manuscript; available in PMC: 2020 Jan 18.
Published in final edited form as: ACS Chem Biol. 2018 Dec 26;14(1):50–57. doi: 10.1021/acschembio.8b00872

Figure 5.

Figure 5.

Effect of partial RNAi knockdown of pdha-1 on indole-containing metabolites. a) Partial RNAi (pdha-1) strongly reduces IAA levels, moderately reduces ICA levels, and strongly increases IPA levels in the worm. [2H5]-IAA for GC-MS and [2H5]-IPA for LC-MS was spiked into samples to control for differences in extraction efficiencies across samples. Data represent the mean ± SD of five independent experiments. Examples are shown of GC-MS traces used in the analysis of IAA (b) and ICA (c) and LC-MS traces used in the analysis of IPA (d). These traces are representative of those traces that were analyzed to generate the graph in (a).