Figure 4. CCR2 mediates airway monocyte recruitment following influenza infection.

Mice were infected with PR8 and seven days later super-challenged with 4×10⁸ CFU MRSA or PBS control. At 24 h BALF cells from WT and Ccr2^−/− mice were analyzed for (A) airway myeloid cell profiles (mean of 4–5 mice/group), (B) compositions of CD11c^–CD11b⁺ inflammatory cells (mean of 4–5 mice/group), and (C-D) numbers (mean±SEM) of CD11b⁺Ly6C⁺ monocytes (Mo) (C), and CD11b⁺Ly6G⁺ neutrophils (PMN) (D). P<0.05, two-way ANOVA; *P< 0.05, **P< 0.01, ***P< 0.001, Tukey’s multiple comparisons test. All mice were treated with gentamicin 4 h after MRSA infection. Data shown are representative of two independent experiments.