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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Immunol. 2019 Feb 20;202(7):2005–2016. doi: 10.4049/jimmunol.1800985

Figure 3. Vaccination with YopB/LcrV+dmLT induces systemic and mucosal IgG and IgA antibody secreting cells (ASC) and antibodies.

Figure 3.

Adult mice were immunized as described in Fig. 1B. Spleen and bone marrow were collected four weeks after the last immunization. Lung tissue and Bronchoalveolar lavage fluid (BALf) were collected one week after the last immunization. YopB, LcrV and dmLT-specific IgG and IgA ASC were measured by ELISpot. Graphs depict ASC counts per 106 cells (mean from duplicate wells) measured in 5 individual mice in each group for YopB and LcrV and in pooled tissue from the same 5 mice (in duplicate) for dmLT. Antigen-specific IgG and IgA were also measured by ELISA in BALf. Data correspond to individual titers from 5 mice per group. Mean response is shown as a line. Significant differences were determined by 2-way ANOVA with Dunnet’s multiple comparisons test; *, P<0.05 compared to PBS.