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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Sep 19;143(4):1426–1443.e6. doi: 10.1016/j.jaci.2018.08.042

Figure 4. IL-1α signaling is required for skin inflammation in ft/ft mice.

Figure 4.

On day 21, ft/ft mice with skin inflammation were treated with PBS (ft/ft control) or IL-1Ra twice daily for 7 days (A-D) or with isotype control mAb (ft/ft control), anti-IL-1β mAb, or anti-IL-1α mAb (E-H) and skin samples collected on day 28. (A, E) Representative histology (H&E) (scale bar = 200 μm). (B, F) Mean epidermal thickness (μm) ± s.e.m. (C, G) Representative immunofluorescence of sections (400×) labeled with anti-IL-1α (green, upper panels) and DAPI (blue, lower panels) with merged labeling (cyan, lower panels). Dashed line = dermoepidermal junction and insets = 4× higher digital magnification of the nuclei. (D, H) Mean number ± s.e.m. of PMNs, monocytes and eosinophils per million cells from day 28 skin. *P<0.05, ‡P<0.001, vs. ft/ft control, as calculated by two-tailed Student’s t-test. Results are representative of 2 independent experiments (n = 4–5 mice/group per experiment).