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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Mol Neurobiol. 2018 Sep 19;56(6):3948–3957. doi: 10.1007/s12035-018-1349-5

Fig. 2.

Fig. 2

Specificity of L-type calcium channel blockade. A, βIII-tubulin immunostaining of cortical cultures after five days of regeneration under the indicated conditions. B, Quantification of axon regeneration. Verapamil (L-type calcium channel blocker) significantly enhances axon regeneration in the presence of CSPG. Inhibitors of other calcium channel sub-types (ω-conotoxin MVIIA, N-type; SNX-482, R-type; NNC 55-0396, T-type) do not affect cortical axon regeneration at the indicated concentrations. Data are mean ± S.E. n = 6-29. Scale bar, 100 μm. ***, p < 0.001 vs. CSPG control, one-way ANOVA, post hoc Dunnett.