Concentration-responses for L-type calcium channel blockers combined with Y27632. A, βIII-tubulin immunostaining of cortical cultures after five days of regeneration under the indicated conditions (Y27632, 25 μM; diltiazem, 5 μM). B, Quantification of axon regeneration. Data are mean ± S.E. n = 5-12. Scale bar, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. CSPG control, one-way ANOVA, post hoc Dunnett.