Figure 3.

Activities of Mutational Processes in Human Cancer Cells

(A–C) Bars represent the numbers of base substitutions attributed to mutational signatures (patterns in Figure S1) and indels in stock cell lines (A; cancer type abbreviations in Table S2) and their respective parent (B) and daughter or granddaughter clones (C), which were acquired during the indicated time frames following the experimental design in Figure 2. Daughter clones were cultivated for the numbers of days indicated in brackets. Mutational signatures are ordered and colored according to the associated etiologies. Ins/del - rep/micro/other, small insertions/deletions at repetitive regions, microhomology-mediated or other; complex, complex indels.

‡ Only single parent clones from HT-115, LS-180, and AU565 cell lines were subject to whole-genome sequencing, and their sequences were used as proxies for the mutational catalogs of the corresponding stock cell lines (STAR Methods). The high number of mutations in ESS-1 B1a clone is likely due to its establishment from two cells (Figure S7). Daughters were not successfully established from SNU-81_B parent clone.