Figure 6.

Mutational Signatures in Single Cells Indicate Commonly Continuing APOBEC-Associated Mutagenesis

(A) Bars represent the numbers of base substitutions (left) and mutational signatures (right) in whole-genome-sequenced stock cell lines and their single cells (cancer type abbreviations in Table S2). Mutations presenting at <50% VAF (variant allele fraction) were excluded from mutational catalogs from single cells, as they mostly formed patterns of signatures scE and scF, likely introduced during the process of the single-cell DNA preparation (STAR Methods; see Figure S6A for mutational signatures annotation using complete catalogs).

(B) Mutational signatures extracted from the complete mutational catalogs from 36 whole-genome-sequenced single cells. Each signature is displayed according to the 96-substitution classification on horizontal axis, defined by the six color-coded substitution types and sequence context immediately 5′ and 3′ to the mutated base. Vertical axes show the percentage of mutations attributed to specific mutation types.