Figure 3.

CpG stimulation reduces antigen uptake capacity of MPs and, after a prolonged treatment, downregulates surface MHCII expression. MPs were either left untreated or stimulated with 2μM CpGs for 1 and 7 days, as indicated. The cells were incubated with 10 μg/ml of Ova conjugated with AlexaFluor-488 for 1 h prior to harvesting and analysis using flow cytometry. The numbers in the individual histograms show the mean fluorescence intensities of the stained samples (black contours). The non-stained samples are represented with filled gray contours. Representative results from two experiments with cells from different individuals are shown.