Table 1.
Kinetics of hSR mutants highlighting the S84D mutants switch in preference to elimination of serine over charged substrates and the interesting preference of S84T for L-SOS over L-THA [table adapted with permission of the American Society for Biochemistry and Molecular Biology (ASBMB) (Nelson et al., 2017)].
| kcat/Km (Sub.): kcat/Km (L-Ser) | |||
|---|---|---|---|
| Variant | L-SOS | L-THA | L-Ser vs. L-SOS vs. L-THA |
| WT | 100:1 | 93:1 | 1:100:93 |
| S84A | 71:1 | 34:1 | 1:71:34 |
| S84D | 1:12 | 1:50 | 50:4:1 |
| S84N | 2.5:1 | 7:1 | 1:2.5:7 |
| S84T | 370:1 | 50:1 | 1:370:50 |