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. 2019 Mar 19;9:4865. doi: 10.1038/s41598-019-41378-9

Figure 3.

Figure 3

The PNGase F immobilization plasmid construction and maintenance in E. coli cells, and phage yield of the immobilization procedure. (A) Schematic of the PNGase F immobilization plasmid. The circular plasmid DNA molecule is represented by a circle. The PNGase F and Soc genes are represented by green ribbons and the expression directions are indicated as arrow shapes. Soc promoter, Soc terminator, ampicillin antibiotic resistance gene (bla), and the replication origins are indicated. (B) Screening of the transformants for the presence of the PNGase F-Soc ORF. Transformants 1–10 were subjects for colony PCR with primers specific to the PNGase F-Soc ORF, and the PCR products were analyzed by agarose gel electrophoresis. PCR using the PNGase F immobilization plasmid as the template served as the positive control. PCR without a template was used as the negative control. (C) Phage yield of the immobilization by self-assembly procedure. The number of phage particles produced from one E. coli cell is presented in plaque forming units (p.f.u.). Phage yields from 6 independent experiments (bars 1–6) are shown. The exact number of phage yield of each experiment is indicated on top of each bar.