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. 2019 Mar 19;9:4853. doi: 10.1038/s41598-019-41269-z

Figure 1.

Figure 1

Targeting constructs and PCR strategies. Schematic depiction of (a) the original targeting construct and (b) targeting variants in the Anp32b locus with PCR oligonucleotide primers allowing detection of the respective wild type allele, “floxed” allele and “Cre’ed” allele. (c) Targeting variants of Anp32b transcripts using PCR primers for detecting Anp32b knockout. FRT, flippase (Flp) target site; loxP, Cre target site; En2 SA, engrailed homeobox 2 splice acceptor; T2A, self-cleaving 2A peptide sequence; lacZ, β-galactosidase reporter gene; neo, neomycin resistance gene; pA, polyadenylation signal.