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. 2019 Mar 19;9:4853. doi: 10.1038/s41598-019-41269-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of Anp32b KO in mice. (a) Immunohistochemical detection of Anp32b in spleens. Spleen sections from Anp32b KO and wild type (WT) animals were stained with a monoclonal antibody specific for murine Anp32b. (b) Detection of Anp32b by Western blot analysis. Single cell suspensions of murine splenocytes from Anp32b KO and WT animals were prepared and crude protein extracts were analyzed using polyclonal ANP32B antiserum.