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. 2019 Mar 19;9:4853. doi: 10.1038/s41598-019-41269-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hematopoietic characterization of Anp32b KO mice. Splenic single cell suspensions from naïve Anp32b KO and WT animals were analyzed for cell counts, viability, T lymphocyte prevalence, activation status and differentiation. (a) Splenic T and B cell lymphocytes were analyzed based on CD3 and B220 expression. (b) CD3⁺ peripheral T cell subsets were analyzed with respect to their expression of CD4 and CD8 surface molecules. (c) The activation status of splenic CD4⁺ or (d) CD8⁺ peripheral T cells as well as the activation status of (e) B220⁺ B cells was further analyzed with respect to naive and effector memory lymphocyte subsets of the spleen. (f) Splenic cells were further analyzed for percentages of CD4⁺CD25⁺Foxp3⁺ T_regs and CD4⁺CD25⁺Foxp3⁻ within the CD4⁺ population. Data are mean ± SD. Anp32b WT (n = 4) and Anp32b KO (n = 4). *p < 0.05; Mann-Whitney U test.