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. 2019 Mar 19;9:4853. doi: 10.1038/s41598-019-41269-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased Th17 CD4⁺ T cell population in Anp32b KO mice. Splenic CD4⁺ T cells of Anp32b KO mice and controls were incubated for 4–6 h with 1 mg/ml ionomycin, 20 ng/ml PMA, and 1 mg/ml Brefeldin A (BD GolgiPlug). Cells were stained to determine the percentage of Th1, Th2 and Th17 cells among the CD4⁺ cell population. Anp32b wild type (WT; n = 6) and Anp32b KO (n = 7). Pooled data (mean ± SEM) from two independent experiments are shown. *p < 0.05, **p < 0.01, Mann-Whitney U test was used.